The Sekelsky Lab

pP{Target}

pP{Target} was built by Sarah Radford as part of her summer research. It is based on pCaSpeR, but has 34-bp FRT sites, an I-CreI endonuclease site, and chromatin insulators to protect against position effects. Unfortunately, the insulators are probably too short to actually work - we get the entire range of eye colors among different transformant lines. pP{TargetB} is similar, but without the insulators, making it somewhat smaller.

pP{Target} Sequence

CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTT GCAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGAC AGCGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAAT ATTAATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAG ctagAGTTGTTGGTTGGCACACCACAAATATACTGTTGCCGAGCACAATTGATCGGCTA AATGGTATGGCAAGAAAAGGTATGCAATATAATAATCTTTTATTGGGTATGCAACGAAA ATTTGTTTCGTCAACGTATGCAATATTTTTTATTAAAAGAGGGTATGCAATGTATTTTA TTAAAAACGGGTATGCAATAAAAAATTATTTGGTTTCTCTAAAAAGTATGCAGCACTTA TTTTTTGATAAGGTATGCAACAAAATTTTACTTTGCCGAAAATATGCAATGTTTTTGCG AATAAATTCAACGCACACTTATTACGTGGCCggccgctcgacGAAGTTCCTATTCTCTA GAAAGTATAGGAACTTCggatccAGATCTGCTAGCGGCCGCAGGCCTACGCGTGGTACC GAATTCtagtatgtatgtaagttaataaaacccatttttgcggaaagtagataaaaaaa acattttttttttttactgcactggatatcattgaacttatctgatcagttttaaattt acttcgatccaagggtatttgatgtaccaggttctttcgattacctctcactcaaaatg acattccactcaaagtcagcgctgtttgcctccttctctgtccacagaaatatcgccgt ctctttcgccgctgcgtccgctatctctttcgccaccgtttgtagcgttacctagcgtc aatgtccgccttcagttgcactttgtcagcggtttcgtgacgaagctccaagcggttta cgccatcaattaaacacaaagtgctgtgccaaaactcctctcgcttcttatttttgttt gttttttgagtgattggggtggtgattggttttgggtgggtaagcaggggaaagtgtga aaaatcccggcaATGGGCCAAGAGGATCAGGAGCTATTAATTCGCGGAGGCAGCAAACA CCCATCTGCCGAGCATCTGAACAATgtgagtagtacatgtgcatacatcttaagttcac ttgatctataggaactgcgattgcaacatcaaattgtctgcggcgtgagaactgcgacc cacaaaaatcccaaaccgcaatcgcacaaacaaatagtgacacgaaacagattattctg gtagctgtgctcgctatataagacaatttttaagatcatatcatgatcaagacatctaa aggcattcattttcgactacattcttttttacaaaaaatataacaaccagatattttaa gctatgcacaaaaaataaataaaagtataaacctacttcgtaggatacttcgttttgtt cggggttagatgagcataacgcttgtagTTGATATTTGAGATCCCCTATCATTGCAGGG TGACAGCGGACGCTTCGCAGAGCTGCATTAACCAGGGCTTCGGGCAGGCCAAAAACTAC GGCACGCTCCTGCCACCCAGTCCGCCGGAGGACTCCGGTTCAGGGAGCGGCCAACTAGC CGAGAACCTCACCTATGCCTGGCACAATATGGACATCTTTGGGGCGGTCAATCAGCCGG GCTCCGGATGGCGGCAGCTGGTCAACCGGACACGCGGACTATTCTGCAACGAGCGACAC ATACCGGCGCCCAGGAAACATTTGCTCAAGAACGgtgagtttctattcgcagtcggctg atctgtgtgaaatcttaataaagggtccaattaccaatttgaaactcagTTTGCGGCGT GGCCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCC TGCTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATG CGACTGCTCAATGGCCAACCTGTGGACGCCAAGGAGATGCAGGCCAGGTGCGCCTATGT CCAGCAGGATGACCTCTTTATCGGCTCCCTAACGGCCAGGGAACACCTGATTTTCCAAG CCATGGTGCGGATGCCACGACATCTGACCTATCGGCAGCGAGTGGCCCGCGTGGATCAG GTGATCCAGGAGCTTTCGCTCAGCAAATGTCAGCACACGATCATCGGTGTGCCCGGCAG GGTGAAAGGTCTGTCCGGCGGAGAAAGGAAGCGTCTGGCATTCGCCTCCGAGGCACTAA CCGATCCGCCGCTTCTGATCTGCGATGAGCCCACCTCCGGACTGGACTCATTTACCGCC CACAGCGTCGTCCAGGTGCTGAAGAAGCTGTCGCAGAAGGGCAAGACCGTCATCCTGAC CATTCATCAGCCGTCTTCCGAGCTGTTTGAGCTCTTTGACAAGATCCTTCTGATGGCCG AGGGCAGGGTAGCTTTCTTGGGCACTCCCAGCGAAGCCGTCGACTTCTTTTCCTAgtga gttcgatgtgtttattaagggtatctagcattacattacatctcaactcctatccagCG TGGGTGCCCAGTGTCCTACCAACTACAATCCGGCGGACTTTTACGTACAGGTGTTGGCC GTTGTGCCCGGACGGGAGATCGAGTCCCGTGATCGGATCGCCAAGATATGGCGACAATT TTGCCATTAGCAAAGTAGCCCGGGATATGGAGCAGTTGTTGGCCACCAAAAATCTGGAG AAGCCACTGGAGCAGCCGGAGAATGGGTACACCTACAAGGCCACCTGGTTCATGCAGTT CCGGGCGGTCCTGTGGCGATCCTGGCTGTCGGTGCTCAAGGAACCACTCCTCGTAAAAG TGCGACTTATTCAGACAACGgtgagtggttccagtggaaacaaatgatataacgcttac aattcttggaaacaaattcgctagattttagatagaattgcctgattccacacccttct tagtttttttcaatgagatgtatagtttatagttttgcagAAGATAAATAAATTTCATT TAACTCGCGAATATTAATGAGATGCGAGTAACATTTTAATTTGCAGATGGTTGCCATCT TGATTGGCCTCATCTTTTTGGGCCAACAACTCACGCAAGTGGGTGTGATGAATATCAAC GGAGCCATCTTCCTCTTCCTGACCAACATGACCTTTCAAAACGTCTTTGCCACGATAAA Tgtaagtcatgtttagaatacatttgcatttcaataatttactaactttctaatgaatc gattcgatttagGTGTTCACCTCAGAGCTGCCAGTTTTTATGAGGGAGGCCCGAAGTCG ACTTTATCGCTGTGACACATACTTTCTGGGCAAAACGATTGCCGAATTGCCGCTTTTTC TCACAGTGCCACTGGTCTTCACGGCGATTGCCTATCCGATGATCGGACTGCGGGCCGGA GTGCTGCACTTCTTCAACTGCCTGGCGCTGGTCACTCTGGTGGCCAATGTGTCAACGTC CTTCGGATATCTAATATCCTGCGCCAGCTCCTCGACCTCGATGGCGCTGTCTGTGGGTC CGCCGGTTATCATACCATTCCTGCTCTTTGGCGGCTTCTTCTTGAACTCGGGCTCGGTG CCAGTATACCTCAAATGGTTGTCGTACCTCTCATGGTTCCGTTACGCCAACGAGGGTCT GCTGATTAACCAATGGGCGGACGTGGAGCCGGGCGAAATTAGCTGCACATCGTCGAACA CCACGTGCCCCAGTTCGGGCAAGGTCATCCTGGAGACGCTTAACTTCTCCGCCGCCGAT CTGCCGCTGGACTACGTGGGTCTGGCCATTCTCATCGTGAGCTTCCGGGTGCTCGCATA TCTGGCTCTAAGACTTCGGGCCCGACGCAAGGAGTAGCCGACATATATCCGAAATAACT GCTTGTTTTTTTTTTTTACCATTATTACCATCGTGTTTACTGTTTATTGCCCCCTCAAA AAGCTAATGTAATTATATTTGTGCCAATAAAAacaagatatgacctatagaatacaagt atttccccttcgaacatccccacaagtagactttggatttgtcttctaaccaaaagact tacacacctgcataccttacatcaaaaactcgtttatcgctacataaaacaccgggata tattttttatatacatacttttcaaatcgcgcgccctcttcataattcacctccaccac accacgtttcgtagttgctctttcgctgtctcccacccgctctccgcaacacattcacc ttttgttcgacgaccttggagcgactgtcgttagttccgcgcgattcggttcgctcaaa tggttccgagtggttcatttcgtctcaatagaaattagtaataaatatttgtatgtaca atttatttgctccaatatatttgtatatatttccctcacagctatatttattctaattt aatattatgactttttaaggtaattttttgtgacctgttcggagtgattagcgttacaa tttgaactgaaagtgacatccagtgtttgttccttgtgtagatgcaACCAAACTGTCTC ACGACGTTTTGtgcatacacattattttgtgtgtatatggtgacgtcacaactactaag caataagaaattttccagacgtggctttcgtttcaagcaacctactctatttcagctaa aaataagtggatttcgttggtaaaatacttcaattaagcaaagaactaactaactaata acatgcacacaaatgctcgagtcgacGAAGTTCCTATTCTCTAGAAAGTATAGGAACTT CggatccgcggccggccactagccggactagaaagtataggaacttcggatctagaGTT GTTGGTTGGCACACCACAAATATACTGTTGCCGAGCACAATTGATCGGCTAAATGGTAT GGCAAGAAAAGGTATGCAATATAATAATCTTTTATTGGGTATGCAACGAAAATTTGTTT CGTCAACGTATGCAATATTTTTTATTAAAAGAGGGTATGCAATGTATTTTATTAAAAAC GGGTATGCAATAAAAAATTATTTGGTTTCTCTAAAAAGTATGCAGCACTTATTTTTTGA TAAGGTATGCAACAAAATTTTACTTTGCCGAAAATATGCAATGTTTTTGCGAATAAATT CAACGCACACTTATTACGTGGCctgcagcatgcgtttaaacctagtaattaattcggct gctgctctAAACGACGCATTTCGTACTCCAAAGTACGAATTTTTTCCCTCAAGCTCTTA TTTTCATTAAACAATGAACAGGACCTAACGCACAGTCACGTTATTGTTTACATAAATGA TTTTTTTTACTATTCAAACTTACTCTGTTTGTGTACTCCCACTGGTATAGCCTTCTTTT ATCTTTTCTGGTTCAGGCTCTATCACTTTACTAGGTACGGCATCTGCGTTGAGTCGCCT CCTTTTAAATGTCTGACCTTTTGCAGGTGCAGCCTTCCACTGCGAATCATTAAAGTGGG TATCACAAATTTGGGAGTTTTCACCAAGGCTGCACCCAAGGCTCTGCTCCCACAATTTT CTCTTAATAGCACACTTCGGCACGTGAATTAATTTTACTCCAGTCACAGCTTTGCAGCA AAATTTGCAATATTTCATTTTTTTTTATTCCACGTAAGGGTTAATGTTTTCAAAAAAAA ATTCGTCCGCACACAACCTTTCCTCTCAACAAGCAAACGTGCACTGAATTTAAGTGTAT ACTTCGGTAAGCTTCGGCTTTCGACGGGACCACCTTATGTTATTTCATCATGggccaga cccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccaggatgacctt gcccgaactggggcacgtggtgttcgacgatgtgcagctaatttcgcccggctccacgt ccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagaggtacgac aaccatttgaggtatactggcaccgagcccgagttcaagaagaaggcgtttttccatag gctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacc cgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggc gctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagc tgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactat cgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaa caggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggccta actacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttacc ttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgg tttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctt tgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttg gtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttt taaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactcccc gtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgat accgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaa gggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgt tgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccat tgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggtt cccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcc ttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttat ggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactg gtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgc ccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcat tggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagtt cgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtt tctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacg gaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggtt attgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtt ccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgac attaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatg acggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcg gatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcgggg ctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtg aaataccgcaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtcgaacaaaa ggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggt gtggtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaata tatcccggtgttttatgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgta agtcttttggttagaagacaaatccaaagtctacttgtggggatgttcgaaggggaaat acttgtattctataggtcatatcttgtttttattggcacaaatataattacattagctt tttgagggggcaataaacagtaaacacgatggtaataatggtaaaaaaaaaaacaagca gttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccagat atgcgagcacccggaagctcacgatgagaatggccagac

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